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KMID : 0613820090190030349
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Journal of Life Science
2009 Volume.19 No. 3 p.349 ~ p.355
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Comparison of Metabolic Fingerprintings between Biofilm and Aeration Tanks of RABC Systemfor Food Wastewater Treatment
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Lee Dong-Geun
Lee Sang-Hyeon
Lee Jae-Hwa
Park Seong-Joo
Ha Bae-Jin
Ha Jong-Myung
Yoo Ki-Hwan
Sung Gi-Moon
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Abstract
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Metabolic fingerprinting of microbial communities was investigated with Biolog GN2 plates using samples of biofilm and aeration tanks from an RABC (rotating activated Bacillus contactor) system - an advanced wastewater treatment system for the food wastewater of pig slaughterhouses. Aerobic and anaerobic results revealed the following four aspects. First, simple matching and pairs t-test of daily variation showed more defined qualitative and quantitative relatedness of active microbial communities than that of mere optical densities. Second, metabolic potentials were higher in biofilm than in aeration tanks (p＜0.01), meaning higher activity of biofilm. Third, two aeration tanks showed the highest similarity (78%) and similar metabolic power (p=0.287). However, actively used carbon sources were different among samples, signifying change of active communities at each wastewater treatment step. Finally, aerobic and anaerobic metabolic fingerprinting patterns were different for the same samples representing activities of microaerophilic and/or anaerobic communities. These results suggest that daily variation and anaerobic incubation would help in the comparison of metabolic fingerprintings.
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KEYWORD
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Metabolic fingerprinting, biofilm, Biolog, RABC
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